The 1-0 defeat on Friday dealt a blow to Leicester City’s hopes of securing automatic promotion to the Premier League.
Despite starting the game, Wilfred Ndidi was substituted for Dennis Praet in the second half as Leicester tried to overturn the scoreline.
Mustapha Bundu’s decisive goal secured Plymouth their first home win since January, condemning Enzo Maresca’s side to back-to-back defeats following their midweek loss to Millwall.
With only two wins in their last six games, Leicester dropped from the top spot, and were overtaken Ipswich.